Comparison of fibrinogen concentration by Clauss method and by prothrombin-time-derived method in an automated coagulometer
DOI:
https://doi.org/10.35954/SM2024.43.2.7.e301Keywords:
fibrinogen, indicators and reagents, laboratories, blood coagulation tests, international normalized ratio, clinical laboratory techniques, plasma, prothrombin time, reagent stripsAbstract
Introduction: Fibrinogen is essential in hemostasis and clot formation. There are several methods for its quantification, being the Clauss method the recommended one due to its precision, as opposed to the method derived from prothrombin time, which tends to overestimate the values.
The purpose of this study is to compare both methods to corroborate whether there are significant differences in fibrinogen results in patients at the Hospital Central de las Fuerzas Armadas.
Materials and Methods: 153 patient samples were analyzed, divided into three groups:
Group 1: Elevated fibrinogen (>450 mg/dL) and normal remaining crassis.
Group 2: INR >1.4 and elevated fibrinogen.
Group 3: low fibrinogen (<200 mg/dL).
The results of derived fibrinogen and Clauss method were compared by Passing-Bablok, Deming and Bland Altman regression.
Results: Derived fibrinogen showed a positive bias in all groups. Although a linear relationship between both methods was confirmed, Bland Altman analysis indicated statistically significant differences (p<0.0001). The bias was 33% for groups 1 and 3; and 26% for group 2.
Discussion and Conclusion: The derived method overestimates fibrinogen levels, which can lead to erroneous diagnoses, particularly in patients with low or high levels. The Clauss method is more accurate and reliable, and should be preferred for patients with critical alterations such as hypofibrinogenemia or dysfibrinogenemia and for its correct use as a marker of systemic inflammation.
This article was approved by the Editorial Committee.
Received for review: March 2024.
Accepted for publication: June 2024.
Correspondence: Av. 8 de Octubre 3020, CP 11600, Montevideo, Uruguay. Tel: +598 24876666 int. 6457.
Contact e-mail: mtesturi@dnsffaa.gub.uy.
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