99mTc-HYNIC-Fab(Bevacizumab): potential imaging agent for diagnosis of Non-Hodgkin's Lymphoma
DOI:
https://doi.org/10.35954/SM2017.36.2.2Keywords:
Lymphoma, Non-Hodgkin, VEGF, Fab 99m (Bevacizumab), Tc, HYNIC, Tumor AngiogenesisAbstract
Vascular endothelial growth factor (VEGF) is a key factor in tumor angiogenesis in many types of tumors, including non-Hodgkin's lymphoma (NHL). The aim of the present work is the radiolabeling of antigen binding fragments (Fab) of the 99m monoclonal antibody Bevacizumab with Tc and its evaluation as a potential imaging agent for NHL. To achieve this, VEGF expression was analyzed by flow cytometry in a NHL cell line (Toledo). Fragmentation was performed using papain and the Fab obtained were conjugated with NHS-HYNIC-Tfa and 99m radiolabeled with Tc. Radiochemical purity and stability were assayed. Biodistribution studies were performed in both healthy and NHL-bearing mice. Toledo cells were found to have elevated VEGF expression. Radiolabeling was performed in a rapid, simple, reproducible and stable manner, with radiochemical purities >90%. Biodistribution studies revealed significant renal and tumor uptake, indicating that the main route of elimination is renal. From the 99m results obtained we conclude that Tc-HYNIC-Fab (Bevacizumab) represents a potential imaging agent for VEGF expression associated with NHL.
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