Radionuclide tagging of biomolecules.
DOI:
https://doi.org/10.35954/SM2024.43.2.4.e402Keywords:
Technetium/ administration & dosage., lutecio/administration & dosage, isotope labeling, chelating agents, radiation, radioisotopes, technetium/ administration & dosageAbstract
Introduction: The labeling of biomolecules with radionuclides is a fundamental technique in nuclear medicine that allows the visualization and treatment of various diseases, especially cancer.
Materials and methods: This work discusses in detail the methods and applications of this technology, including radionuclide selection, conjugation to biomolecules using chelating agents such as dodecane tetraacetic acid (DOTA), Diethylene Triamino Penta Acetic Acid (DTPA), Hydrazinonicotinic acid (HYNIC), and purification and characterization processes.
Results: Specific examples are presented, such as the labeling of Bevacizumab and Tocilizumab with 99mTc and 177Lu, highlighting their applications in cancer imaging and therapy. In addition, clinical applications and future research in the field are explored, emphasizing the potential for personalized medicine and combination therapies.
Discussion: Radionuclide tagging technology continues to advance, promising significant improvements in the diagnosis and treatment of complex diseases, offering accurate and efficient tools for healthcare management. This chapter provides a comprehensive overview of the techniques, case studies and the impact of this methodology in clinical practice.
This article was approved by the Editorial Board.
Received for review: March 2024.
Accepted for publication: June 2024.
Correspondence: Nuclear Research Center. Mataojo 2055. CP. 11400, Montevideo, Uruguay. Tel: 598(2)99472572.
Contact e-mail: pcabral@cin.edu.uy
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